Thp1 doubling time

Author: biiu

August undefined, 2024

WebDoubling time: ca. 40-50 hours : Harvest: saturation density at about 1.0 x 10 6 cells/ml : Storage: frozen with 70% medium, 20% FBS, 10% DMSO : DSMZ Scientific Data: Mycoplasma: initial contamination was eliminated with Ciprobay (ciprofloxacin), thereafter negative in DAPI, microbiological culture and repeatedly in PCR assays : Immunology: http://www.bowdish.ca/lab/wp-content/uploads/2011/07/THP-1-propagation-culture.pdf

MLL-AF9 oncogene expression affects cell growth but not

Web1. To revive THP-1 and Jurkat cells from frozen liquid N 2 stocks, warm RPMI-1640 supplemented with FBS (10%, complete media) to 37 °C in a water bath.. 2. Thaw the … WebJul 1, 2016 · 1. Thaw the GF-AFC substrate and Assay Buffer components from the CellTiter-Fluor™ Cell Viability Assay kit following the detailed procedure in the Technical Bulletin #371 ( 32 ). 2. Transfer 10 µl of the GF-AFC Substrate into 10 ml of the Assay Buffer to prepare a 2X Reagent. pack magnate moderno xbox

Cellosaurus cell line THP-1 (CVCL_0006)

WebAspirate and add fresh culture medium every 2-3 days. HepG2 cell doubling time is 48 hours. To passage cells, rinse cell monolayer with 1x PBS twice and add pre-warmed (37°C) 0.05% Trypsin-EDTA solution to cover the bottom of the flask; incubate for 5 – 7 minutes. As cells detach, neutralize the Trypsin by adding 4x volume of complete growth ... WebJun 17, 2009 · For FaDu cells a doubling time of 22 hours and for THP-1 cells of 38 hours could be observed in logarithmic phase. For all experiments with CKIA and CKIB tumor cells were in logarithmic growth phase. After 72 hours of incubation with TPA, THP-1 monocyte-like suspension cells adhere almost completely at the bottom of culture flask and … WebMay 8, 2015 · Cell Line Name Panel Name Doubling Time Inoculation Density; LXFL 529: Non-Small Cell Lung : 10000: DMS 114: Small Cell Lung : 20000: SHP-77: Small Cell Lung : 40000 packman compression fitting

Adaptation of a human monocytic leukemia cell line (THP-1) in

What is the growth rate of THP1 cell line? ResearchGate

WebSep 27, 2024 · Ushashi Banerjee. 1) Thawing the vial in 37C water bath, then spinning down at ~800rpm for 3 minutes. Then decant the FBS, resuspend in pre-warmed media and gave another spin. Then I resuspended ... WebMay 11, 2015 · This is an important aspect as it removes the presence of any phagocytic aspect to changes in the cancer cell number and behaviour. The in vitro THP-1 monocyte … いわき市税金高いWebTHP-1 is a human leukemia monocytic cell line, which has been extensively used to study monocyte/macrophage functions, mechanisms, signaling pathways, and nutrient and drug transport. This cell line has become a common model to estimate modulation of monocyte and macrophage activities. This review a … いわき市窓リフォーム

"WebTHP-1 cells are a human monocyte suspension cell line from peripheral blood of a 1 year old infant who had acute mnocytic leukemia. " - Thp1 doubling time

Thp1 doubling time

WebApr 4, 2024 · Population doubling times estimated from the MTS assay, manual counting, and volumetric flow cytometer were 18.2, 17.1, and 17.0 hours, respectively, ... 24.5 hours … WebA. DSMZ Cell Culture Data: Morphology: round, single cells in suspension, partly in clusters; image; image; image; image. Medium: 80-90% RPMI 1640 + 10-20% h.i. FBS. Subculture: seed out at ca. 0.5 x 10 6 cells/ml (20% FBS and a 12- or 24-well plate could be of advantage); maintain at 0.2-1.0 x 10 6 cells/ml; split 1:2 to 1:3 every 3-4 days ...

Did you know?

WebThe doubling time of THP1 cells are approximately 26 hrs. The cells are happiest at a density between 1x10E5 cells/ml to 1x10E6 cells/ml. If they … WebTHP-1: Synonyms: THP1; THP 1; THPI; THP-1(ATCC); THP-1-O; O-THP-1; Tohoku Hospital Pediatrics-1: Accession: CVCL_0006: Resource Identification Initiative: To cite this cell line …

WebExo from culture supernatants of non-stimulated THP-1-di erentiated macrophages ranged from 97–467 nm with a mode diameter of 120 nm (Figure2A). More particles (p < 0.0001) were found in the Exo from culture supernatants of LPS-stimulated THP-1 macrophages as compared with those from non-stimulated THP-1 macrophages (Figure2B). The diameter … WebThis cell line was designated as THP-1c12(-). When a sufficient number of THP-1c12(-) cells was seeded in culture, the maximum cell density after 6 days of culture was 1 X 10(6)/ml …

WebProtocol 1. Suspend THP-1 monocytic cells at a concentration of 5 × 10 5 cells/mL in Culture media and aliquot 200 μL to each well of a flat-bottom tissue culture treated 96-well … WebMay 24, 2024 · Under our culture conditions utilizing traditional tissue culture flasks, NK-92 cells grew with a doubling time of 35.6 ± 6.1 h displaying a similar rate of expansion with a prior study using Vuelife culture bags . On the other hand, NK101 exhibited more rapid growth with a doubling time of 21.9 ± 2.4 h.

WebJun 21, 2024 · THP-1 cells were induced from suspension to adherent growth after incubation with PMA. The mRNA levels of macrophage marker CD68 were upregulated (Figure 4B ). To explore whether knockdown of HHLA2 in NSCLC cells was involved in the process of THP-1 macrophage polarization, we transfected A549/H1299 cells with …

WebSOP cell culture_THP-1 1.1 3/11 1 Introduction The THP-1 cell line (ATCC: TIB-202) is a human monocytic cell line derived from the peripheral blood of a one year old human … いわき市窓Although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. In general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their characteristics are: • Expression of Fc receptor and C3b receptors while lacking surface and cytoplasmic immunoglob… いわき市空き家 packlite nova usb solar lanternWebNov 27, 2003 · Time course of MLL-AF9 expression in ATRA (1 μ M)- and PMA (20 n M)-treated THP-1 cells (2.5 × 10 5 cells/ml). At the times indicated, Real-time RT–PCR analysis was performed as described in ... いわき市窓ガラス交換WebHCC827. CRL-2868 ™. HCC827 is an epithelial cell that was isolated from the lung of a White, 39-year-old female patient with adenocarcinoma. This cell line was deposited by AF Gazdar and JD Minna in 1994 and can be used in … packmate definitionWebTHP-1 is a monocyte cell line isolated from the peripheral blood of an acute monocytic leukemia patient and can be used in immunology research. Log In ... Population doubling time Approximately 26 hrs STR profiling. Amelogenin: X,Y. CSF1PO: 11,13. D13S317: 13. … The Credible Conversations blog shares the science and innovation from inside A… Certain materials in the collection may require one or more permits to be filled ou… ATCC is a nonprofit organization that collects, stores, and distributes standard ref… pack mallorcaWebRoswell Park Memorial Institute (RPMI) 1640 medium is a commonly used medium for THP-1 as well as for U937 cells. Alternatively, DMEM (Dulbecco’s Modified Eagle’s Medium; … いわき市管理栄養士パート