Flow cytometry protocol for thp-1 cell

WebProtocol for high-throughput compound screening using flow cytometry in THP-1 cells. Flow cytometry is a valuable method for analyzing protein expressions at the single cell level but can be ... WebMar 18, 2024 · This protocol provides instructions to perform a high-throughput small molecule screen using flow cytometry analysis of THP-1 cells, a human monocytic …

The Identification of Markers of Macrophage Differentiation in …

Web5 Collect 2 million cytotoxic T cells per sample (i.e. per well) from an on-going culture 6 Spin them down at 350 x gfor 00:05:00 at 4 °C and re-suspend them in fresh media at 1 million per mL concentration 7 Supplement T cells with 200 IU/mL rIL2 8 Aspirate the culture media from each of the 24-well-plate wells that contain a sample. Try to aspirate as much as … WebSep 25, 2009 · THP-1 cell surface markers for Flow Cytometry - Need a robust marker for undifferentiated THP-1 cells. I am working with THP-1 cells (ATCC TIB 202), a human monocytic cell line. The majority of people that use this cell line are interested in the differentiated macrophage phenotype, which can be achieved by stimulating THP-1 cells … orb order find the markers 2023 https://daria-b.com

Fc Blocking Controls - Flow Cytometry Guide Bio-Rad

WebJan 13, 2010 · We have compared the phenotype of the promonocytic THP-1 cell line after various protocols of differentiation utilising VD3 and PMA in comparison to primary human monocytes or monocyte-derived macrophages (MDM). ... Flow cytometry was performed to estimate the number of latex beads ingested per cell with each peak assumed to equal … WebJun 5, 2015 · At 24 hpi, IE2-EYFP positive cells were measured by flow cytometry; (b) THP-1 monocytes were differentiated to THP-1 derived macrophages by the addition of PMA for 48 h followed by infection of the obtained differentiated THP-1 cells with TB40E/IE2-EYFP at an MOI of 0.1. At 24 hpi, IE2-positive cells were detected by flow … ipm new york

Flow Cytometry Protocols - Flow Cytometry Guide Bio-Rad / Protocol …

Category:Figure 1. Gating strategy for flow cytometry data from a THP-1...

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Flow cytometry protocol for thp-1 cell

Optimization of the autophagy measurement in a human cell line …

WebSep 1, 2015 · THP-1, a human monocytic cell line and its derived macrophages serve as a useful cell culture model for mechanistic studies of hCMV infection and its underlying biology. A major methodological challenge is the lack of a quick and reliable tool to accurately determine the efficiency of hCMV infection in THP-1 derived macrophages. WebPlease refer to the product webpage and product-specific protocol to determine whether it is compatible with live cell staining. Collect cells by centrifugation and aspirate supernatant. Resuspend cells in 0.5–1 ml 1X PBS. Add formaldehyde to obtain a final concentration of 4%. Fix for 15 min at room temperature.

Flow cytometry protocol for thp-1 cell

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WebCommon cell preparation technique and staining protocols for surface and trapped markers to help get the best quality flow cytometry data. Locate out more. Standard per preparation techniques and coloring protocols for emerge press intracellular markers to assist get the best quality run cytometry data. Find out more WebJan 13, 2024 · Cell Staining and Flow Cytometry Analysis. THP-1 derived M0 and iDC were cultivated in Corning® 96 Well TC-Treated Microplates (Life Sciences, Tewksbury, Massachusetts, USA) with a volume of 100 …

WebAug 8, 2015 · Analysis of CD206 plasma membrane expression by flow cytometry. THP-1 monocytes were seeded in T25 flask at 2.5 × 10 6 cells/T25 and differentiated with PMA. … WebApr 14, 2024 · Cell culture. MOLM13, MV411 and THP-1 cells were obtained from the Sanger Institute cancer cell collection and were grown in 1640-RPMI (Gibco, 21875034) supplemented with 10% FBS (Sigma, F4135 ...

WebRegular cell preparations techniques press staining protocols for surface the cellular markers up helping get the highest quality flow cytometry data. Find output more Gemeinsame cell preparation types and staining record with surface and within markers to help receive one best quality flow cytometry data. WebPublished protocols using the promonocytic cell line THP-1 have tended to result in cells that closely resemble classically-activated macrophages, differentiated in IFNγ and LPS. However, no protocol, to date, has fully recapitulated polarization of THP-1 to the M(IL-4) or M(IL-10) macrophage phenotypes seen when human monocyte-derived ...

WebCell cycle assay protocols for flow cytometry. Vybrant DyeCycle Violet Stain. Vybrant DyeCycle Green and Orange Stains. Vybrant DyeCycle Ruby Stain. FxCycle Violet …

WebApr 4, 2024 · When we compare the level of dye transfer between cells, the CFSE dye in U2OS cells seems to be transferred more to THP-1 cells than the Far Red dye in THP-1 cells being transferred to U2OS cells. To clarify whether the dye transfer is driven by the dyes or cells, we switched the labeling dye and performed another coculture (Fig. 5B). … ipm new lenox ilWebJan 13, 2024 · Cell Staining and Flow Cytometry Analysis. THP-1 derived M0 and iDC were cultivated in Corning® 96 Well TC-Treated Microplates (Life Sciences, Tewksbury, Massachusetts, USA) with a volume of 100 μL per well using the same protocol in the previous section on THP-1 cell Culture and Differentiation. ipm molsheimWebApr 13, 2024 · The recent demonstration of TPI in flow cytometry (TPI-FC) condition 36,37,38 promises to bridge gaps with respect to the conventional imaging flow cytometry systems, i.e. allowing comparable ... ipm not active freightlinerWebMar 9, 2015 · 1.3 For suspension cells, centrifuge to obtain a cell pellet and aspirate the supernatant. Resuspend the cells gently in prewarmed (37°C) probe-containing medium. Incubate the cells for 30 minutes to 2 hours under growth conditions appropriate for the particular cell type (see note above regarding internalization rate of these probes). Re ... ipm new lenoxWebFlow cytometry agreement & procedures including; straightforward staining, directly staining of intracellularly antigen & cytokines, single preparation & permeabilization. 425805 392e8725-8a05-419c-a0a8-99ec1752bb34 ipm nrcsWebIntracellular Staining Flow Cytometry Output Using Detergents to Permeabilize Mobile; Centrifuge cells as by Step 3 and resuspend include appropriate volume in Flow Cytometry Coloring Buffer press buffer of choice so that the final cellular concentration is 1 x 10 7 cells/mL (other cell concentrations may been reasonably for different experiments) orb order in find the markersWebCell cycle assay protocols for flow cytometry. Vybrant DyeCycle Violet Stain. Vybrant DyeCycle Green and Orange Stains. Vybrant DyeCycle Ruby Stain. FxCycle Violet … orb participant app download