Chromotek gfp-trap agarose
WebApr 10, 2024 · GFP-trap agarose beads (gta) were from ChromoTek. Cell lines and culture Human embryonic kidney (HEK) cell lines HEK293T (#CRL-3216) and HEK293E (#CRL-10852) were from ATCC, and MEFs were provided ... WebProteintech ChromoTek GFP-Trap™ Agarose GFP-Trap™ Agarose is an affinity resin for IP of GFP-fusion proteins. Brand: Proteintech GTA-20 Additional Details : Weight : …
Chromotek gfp-trap agarose
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WebApr 10, 2024 · Total cell extracts of yeast cells untreated or treated with CHX were incubated in ChromoTek GFP-Trap Agarose beads (Proteintech) for 2.5 h at 4 °C to pull-down AGP2-GFP tagged-associated proteins. After incubation, the beads coupled to proteins were washed with washing buffer (10 mM Tris/Cl pH 7.5, 0.5 mM EDTA, 150 … WebGFP Nanobody Magnetic Agarose, ChromoTek GFP-Trap® from Proteintech Group Inc This product is no longer available on Biocompare. Product Specs Item GFP Nanobody …
WebThe ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to … WebOct 19, 2024 · While anti-V5-tag antibodies for conventional IP are available from multiple suppliers, ChromoTek is the first to offer anti-V5 Nanobodies bound to agarose beads for immunoprecipitation. V5-Trap is available as agarose or magnetic agarose. Higher purity of V5-Trap vs. conventional IP No contamination with heavy or light chains using V5-Trap.
http://alpaca.chromotek.com/ WebGFP-PFKFB3 was overexpressed in HEK293T cells and immunoprecipitated using GFP-Trap agarose beads. The PFKFB3 complex was then incubated with the indicated amount of recombinant human Src protein for 10 min. Western blot was used to detect tyrosine-phosphorylation level.
WebMay 25, 2024 · ChromoTek GFP-Trap ® is optimized for the immunoprecipitation of GFP-tagged proteins and their interacting factors. The GFP-Trap consists of the ChromoTek anti-GFP Nanobody/ V H H …
WebDora D Robinson, age 70s, lives in Leavenworth, KS. View their profile including current address, phone number 913-682-XXXX, background check reports, and property record … binding financial agreements ipswichWebApr 11, 2024 · Sir2:eGFP was immunoprecipitated with ChromoTek GFP-Trap® Magnetic Agarose (ChromoTek, Planegg, Germany). Elution was performed by boiling the samples in LaemmLi buffer supplemented 200 mM DTT and proteins precipitated by addition of 4 volumes 1:1 methanol:acetone. Protein pellets were resuspended in urea 6 M, 50 mM … cystistat sterile hyaluronate solutionWebThe ChromoTek GFP-Trap® Agarose Kit consists of an anti-Green Fluorescent Protein (GFP) Nanobody (VHH), which is covalently bound to agarose beads. GFP-Trap Agarose Kit is used to immunoprecipitate GFP-fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. binding financial agreement exampleWebBoth the agarose and magnetic bead formats are extremely efficient for immunoprecipitation of GFP tagged proteins, and the usual heavy and … cystistat prospectWebApr 11, 2024 · A 500 μl fraction of each cleared sample was mixed with 50 μl of pre-equilibrated GFP-Trap magnetic agarose (ChromoTek Cat No. gtma) or DYKDDDDK Fab-Trap Agarose (ChromoTek Cat No. ffa) and incubated over-night at 4°C rotating end-over-end. The beads were then washed twice with Wash buffer (10 mM Tris–HCl pH 7.5; 150 … cystistat side effectsWebJan 10, 2024 · The mixture was then incubated with the GFP-Trap Agarose beads (Chromotek, pre-washed by 1 mL extraction buffer) for 4 h at 4 °C. Then, the beads were collected by spinning and washed with the extraction buffer for 3–5 times to eliminate nonspecific bindings. The bound proteins on the GFP-trap were boiled and assayed by … cystisospora sp isospora oocystsWebJun 23, 2024 · Enhanced snoMEN Vectors Facilitate Establishment of GFP-HIF-1α Protein Replacement Human Cell Lines. PLoS ONE. 2016;11:e0154759 pubmed publisher … binding financial agreements perth